Policies of International Cooperation
EJB Electronic Journal of Biotechnology ISSN: 0717-3458
© 1998 by Universidad Católica de Valparaíso -- Chile
BIP INVITED REVIEW ARTICLE

Island communities and biotechnology

Edgar J. DaSilva *
Director Section of Life Sciences Division of Basic Sciences UNESCO
1, Rue Miollis, 75015 Paris France
E-Mail :
e.dasilva@unesco.org

Mary Taylor
Regional Tissue Culture Adviser, Pacific Regional Agricultural Programme
University of the South Pacific Apia Western Samoa
E-Mail:
maryt@samoa.net
Fax: 0685-27035/22933

* Corresponding author

Keywords: Biotechnology, Pacific islands, Caribbean region, MIRCENS

BIP Article

In the run-up to the year 2000 and beyond, several island countries, especially in the Caribbean and pacific regions, are confronted by the challenges and threats of globalisation. In response, several island countries are initiating self-reliant strategies aimed at national and regional endogenous development. Amongst these strategies, the potential of biotechnology for economic development and technological growth is being tapped.


A large number of island communities in the developing world lie in rural or semi-urban areas where agriculture and aquaculture are the main economic activities. The application of biotechnology to these two scientific "cultures" would to a very large extent help revolutionise traditional practices into self-sustaining market ventures that could generate badly needed capital (DaSilva, 1998).


Contemporary research in Asia, Latin America and Africa has already led to the widespread use of the techniques of tissue culture and genetic engineering in maintaining export markets and creating new ones. Current efforts in the Caribbean and pacific regions are indicative of this growing trend.

The Caribbean

The Caribbean nations consist of more than 25 island states with varying populations that depend on a rich diversity of plant genetic resources for their nutrition, health and well-being.

The plan "Caribbean Development to the Year 2000: Challenges, Prospects and Policies" emphasised the general absence of innovation in Caribbean industry. The acquisition of appropriate technology from abroad and the development of a well-defined infrastructure to sustain such technology are identified as effective strategies in responding to these inherent weaknesses. Still again, many Caribbean countries with their island economies based on terrestrial and marine resources are encouraged to reconcile the sustained and judicious development of these resources with climatic and environmental changes. To meet these challenges, and to capitalise on the potential wealth of their resources, several Caribbean Island countries have resorted to the systematic application of biotechnology.

One such step has been the establishment of a Caribbean Biotechnology Network, within the framework of UNESCO's global network of Microbial Resources Centres (MIRCENs) which resulted from political commitment when, a decade ago, the Grenada National Commission for UNESCO, representing the general agreement of Caribbean Ministers responsible for UNESCO Affairs and the Member States in the Caribbean sub-region, resolved that "an extension of the international network of resources centres (MIRCENs) be created in the Caribbean region".

To date, the most common application of biotechnology in the Caribbean islands has been in the fermentation of rum and beer. More recently, tissue culture has been used as an economical tool for the micropropagation of many food and ornamental plants (Table 1). Another recent development is research into methods for food preservation and extension of the shell-life of plant products. The Mona and St. Augustine campuses of the University of the West Indies are involved in joint ventures. For example, the Jamaican Government collaborates with industry in the production of white potatoes, ginger, banana, and yam. In Trinidad, gingerlilies, roses and orchids are produced for local markets. Recently, the technique of embryo transfer has being used to boost meat and milk production.

Table 1
. Current African status and trends in plant biotechnology in Africa.

 Region

Country

Area of Research

North Africa

Egypt

  • Genetic engineering of potatoes, maize and tomatoes

 

Morocco

  • Micropropagation of forest trees, date palms
  • Development of disease-free and stress tolerant plants
  • Molecular biology of date palms and cereals
  • Molecular markers
  • Field tests for transgenic tomato

 

Tunisia

  • Abiotic stress tolerance and disease resistance
  • Genetic engineering of potatoes
  • Tissue culture of date palms, Prunus rootstocks and citrus
  • DNA markers for disease resistance

West Africa

Burkina Faso

  • Biological nitrogen fixation, production of legume inoculants, fermented foods, medicinal plants

 

Cameroon

  • Plant tissue culture of Theobroma cacao (cocoa tree), Hevea brasiliensis (rubber tree), Coffea arabica (coffee tree), Dioscorea spp (yam) and Xanthosoma mafutta (cocoyam)


  • Use of in vitro culture for propagation of banana, oil-palm, pineapple, cotton and tea

 

Cote d'Ivoire

  • In vitro production of coconut palm (Cocos nucifera) and yam
  • Virus-free micropropagation of egg-plant (Solanum spp)
  • Production of rhizobial-based biofertilizers

 

Gabon

  • Large-scale production of virus-free banana, plantain and cassava plantlets

 

Ghana

  • Micropropagation of cassava, banana/plantain, yam, pineapple and cocoa
  • Polymerase Chain Reaction (PCR) facility for virus diagnostics

 

Nigeria

  • Micropropagation cassava, yam and banana, ginger
  • Long term conservation of cassava, yam and banana, and medicinal plants
  • Embryo rescue for yam
  • Transformation and regeneration of cowpea, yam, cassava and Banana
  • Genetic engineering of cowpea for virus and insect resistance
  • Marker assisted selection of maize and cassava
  • DNA fingerprinting of cassava, yams, banana, pests, and microbial pathogens
  • Genome linkage maps for cowpeas, cassava, yams and banana
  • Human resource development through group training, degree related training, fellowships and networking

 

Senegal

  • Well established MIRCEN programme that serves the region of West Africa in microbial-plant interaction

  • Production of rhizobial and mycorhizal-based biofertilizers for rural markets
  • Well established in vitro propagation of Faidherbia albida, Eucalyptus canaldulensis, Sesbania rostrate, Acacia senegal, in co-operation with several international agencies

East & Central Africa

Burundi

  • In vitro production of ornamental plants - orchids, tissue culture of medicinal plants, micropropagation of potato, banana, cassava and yam
  • Supply of disease-free in vitro plants

 

Congo

  • In vitro culture of spinach (Basella alba)
  • Plant pathology - studies in controlling tomato rot due to Pseudomonas solanacearum
  • Bioprospecting of nitrogen-fixing species

 

Congo, Democratic Republic

  • In vitro propagation of potato, soybean, maize, rice and multipurpose trees, e.g. Acacia auriculiforius and Leucaena leucocefhala


  • Production of rhizobial-based biofertilizers in experimental stage
  • Tissue culture of medical plants, e.g. Nuclea latifolia, Phyllanthus niruroides

 

Ethiopia

  • Tissue culture research applied to tef
  • Micropropagation of forest trees

 

Gabon

  • Large-scale production of virus-free banana and plantain (Musa spp) and cassava plantlets (Manihot esculenta)

 

Kenya

  • Production of disease free plants and micropropagationof pyrethrum, bananas, potatoes, strawberries, sweet potato, citrus, sugar cane

  • Micropropagation of ornamentals (carnation, alstromeria, gerbera, anthurium, leopard orchids) and forest trees

  • In vitro selection for salt tolerance in finger millet
  • Transformation of tobacco, tomato and beans
  • Transformation of sweet potato with proteinase inhibitor gene
  • Transformation of sweet potato with Feathery Mottle Virus, Coat protein gene (Monsanto, ISAAA5, USAID6, ABSP7, KARI8)
  • Tissue culture regeneration of papaya
  • In vitro long term storage of potato and sweet potato
  • Marker assisted selection in maize for drought tolerance and insect resistance

  • Well-established MIRCEN providing microbial biofertilizers in the East African region

 

Rwanda

  • Production of rhizobial-based biofertilizers, and Azolla for rice cultivation
  • Tissue culture of medical plants and micropropagation of disease-free potato, banana and cassava

 

Uganda

  • Micropropagation of banana, coffee, cassava, citrus, granadella, pineapple, sweet potato and potato

  • In vitro screening for disease resistance in banana
  • Production of disease free plants of potato, sweet potatoand banana

Southern Africa

Madagascar

  • Tissue culture programme supporting conventional production of disease-free rice and maize plantlets, and Medicinal plants

  • Production of biofertilizers to boost production of Groundnut (Arachis hypogea), bambara groundnut

  • (Vigna subterranea)

 

Malawi

  • Micropropagation of banana, trees (Uapaca), tropical woody species, tea

 

South Africa

  • Genetic engineering
  • -Cereals: maize, wheat, barley, sorghum, millet, soybean, lupins, sunflowers, sugarcane

    -Vegetables and ornamentals: potato, tomato, cucurbits, ornamental bulbs, cassava and sweet potato

    -Fruits: apricot, strawberry, peach, apple, table grapes, banana

  • Molecular marker applications
    -
    Diagnostics for pathogen detection

    -
    Cultivar identification - potatoes, sweet potato, ornamentals, cereals, cassava

    - Seed-lot purity testing - cereals

    - Marker assisted selection in maize, tomato

    - Markers for disease resistance in wheat, forestry crops
  • Tissue culture


  • -Production of disease free plants - potato, sweet potato, cassava, dry beans, banana, ornamental bulbs


    - Micropropagation of potato, ornamental bulbs, rose rootstocks

    -
    chrysanthemum, strawberry, apple rootstocks, endangered species, coffee, banana, avocado, blueberry, date palm

    -Embryo rescue of table grapes, sunflower and dry beans

    -
    In vitro selection for disease resistance - tomato nematodes, guava wilting disease

    - Long term storage - potato, sweet potato, cassava, ornamental bulbs


    - In vitro
    gene bank collections - potato, sweet potato, cassava, ornamentals

    - Forest trees, medicinal plants, indigenous ornamental plants

 

Zimbabwe

  • Genetic engineering of maize, sorghum and tobacco
  • Micropropagation of potato, cassava, tobacco, sweet potato, ornamental plants, coffee
  • Marker assisted selection

 

Zambia

  • Micropropagation of cassava, potato, trees (Uapaca), Banana
  • Hosts SADC9 Nordic-funded gene bank of plant genetic resources

The banana producing and exporting states in the Caribbean region-- Belize, Dominica, Grenada, Jamaica, St. Lucia, St. Vincent and the Grenadines, and the Windward Islands, are plagued by a variety of geographical and economic disadvantages that are uncommon to their Central and South American competitors. Hilly terrain and hurricane-exposed locations add to high management and shipping costs of smaller volumes of banana exports. Crop production on steep terrain and small family venture farms, e.g. in the Windward Islands, continues to be the backbone of several rural economies and the socio-political fabric of a number of the Caribbean island countries. Collapse of such economies, can lead to socio-political turmoil, decimation of the tourist industry, to an exodus of economic refugees, development of undesirable markets e.g. illegal drug trade, and to turbulence and weakening of interdependent socio-economic infrastructures such as CARICOM and the Caribbean Basin Initiative.

In the framework of joint UNDP/UNESCO collaboration on the application of science and technology for development in the Caribbean, research has been carried out on the control of bacterial spot of pepper and tomato caused by Xanthomonas campestris var. vesicatoria (O'Garro, 1997)

The Organisation of the Eastern Caribbean States (OECS), which promotes economic integration and encourages export security in small island countries is composed of Member States - Antigua and Barbuda, Dominica, Grenada, St. Kitts and Nevis, St. Lucia, and St. Vincent and the Grenadines. These island states on account of their monocrop economies, fragile ecosystems, location in the hurricane belt between the Atlantic Ocean and the Caribbean Sea, and little size, are extremely vulnerable to the forces of globalised development. The El Niño phenomenon also has a negative impact on crop production in the Caribbean isles. According to the global information and early warning system on food and agriculture, seasonal cereal and bean crops bosses for 1997 have been estimated at about 20 per cent in several Central American and Caribbean countries. Similar loss risks are anticipated in 1998.

Shock waves have been witnessed in most of the OECS banana-producing countries as a result of the hurricane storms in 1994 and 1995, and still again by the possible loss, after 2001, of European economic preferences for Caribbean bananas. It is estimated that 4000 to 8000 small farmers, in especially Dominica, St. Lucia, and St. Vincent and the Grenadines, may have to exit the banana industry and re-invest in other ventures. In this regard, cultural tourism seems to be a dominating option. Other options for diversification of agricultural markets are to be found in the cultivation of okra, tomatoes and avocados. On the negative side, the allure of easy revenue makes the illegal cultivation of marijuana an attractive proposition despite the risks involved.

Biomethanation programmes in the island states of the Caribbean region have been supported by the Caribbean Development Bank. Jamaica, with funding from the Latin American Energy Organisation (OLADE) has been particularly active in experimenting with a variety of biodigester designs and models (Clancy and Hulscher, 1994).

Caribbean regional health issues and health-care reforms have benefited from IDB/PAHO* (1) collaboration and the Consultative Group for Caribbean Economic Development (CGCED).

Demographic, socio-economic and health indicators in participating countries - Antigua and Barbuda, the Bahamas, Barbados, Belize, Dominica, the Dominican Republic, Grenada, Guyana, Haiti, Jamaica, St. Kitts and Nevis, St. Lucia, St. Vincent and the Grenadines, Trinidad and Tobago, and Suriname, emphasise the need for reducing preventable diseases, and for developing health welfare programmes for aging populations and geriatric diseases. Upward trends have been identified in communicable diseases such tuberculosis and HIV/AIDS, in nutritional deficiencies, and in the re-emergence of malaria, cholera and dengue fever.

The Pacific

Over the past decades, about 27 nations with thousands of islands in the pacific region have emerged with aid through the South Pacific Commission. Like that of the Caribbean, the Pacific region is dependent on a remarkably fragile environment, characterised by limited natural resources, including land, and a high incidence of natural disasters.

The nations of the pacific region share scientific and technological concerns peculiar to their geographic and oceanographic characteristic. They frequently face substantial constraints in terms of scientific, technological, managerial, and economic resources necessary for sustainable, island-wide, maricultural and maritime development. The most important of such developmental and educational issues are microbial aspects related to human health, nutrition, food safety and energy supply.

With the help of the Australian National Commission for UNESCO, Massey University and Cawthron Institute in New Zealand and the government authorities of many of the Pacific island nations, a survey, a decade ago by the MIRCEN at the University of Queensland, Brisbane, established that some 50 researchers from 19 countries in over 44 laboratories were desirous of collaborating with each other.

The South Pacific region is composed of a vast number of islands that vary in size from Papua New Guinea with a population of around four million, to Nuie with a population of 2,000. The diversity of plant genetic resources and the availability of other resources such as labour vary though the latter, as a general rule, is always a limiting factor in agricultural production. Daily subsistence is mainly based on agriculture, though some of the islands have adopted a more commercial approach for export purposes. This is the case in Tonga with its production of squash for the Japanese market. Generally speaking the crops grown throughout the Pacific region are mostly root and tuber crops, banana, and coconut (Table 1).

At the moment, the only real application of biotechnology in the region is tissue culture. It was first introduced into the region through the tissue culture laboratory established by the South Pacific Commission (SPC), Plant Protection Service in Fiji with the aim of increasing the number of varieties of the main root and tuber crops available in the Pacific island countries, and to facilitate their distribution through their pathogen-tested status. In the late 1980s another tissue culture laboratory was established on the campus of the University of the South Pacific (USP), Western Samoa to provide security for the germplasm material that was being maintained in Suva, Fiji.

As with field genebanks throughout the world, losses are occurring at an ever-increasing rate in the South Pacific. Most of the information, on field conservation, which is available for the South Pacific island countries relates to taro and yams. Jackson (1994) recently examined collections of taro and yams established in the 1980s in nine Pacific island countries and found that significant losses have occurred. These have resulted from lack of finances for costly maintenance; inadequate storage facilities, contamination and infection with plant pathogens, and improper protection against cyclonic and drought conditions.

Another area of concern in the South Pacific islands is that of intellectual property rights. Tissue culture projects first became involved with the issues of intellectual property rights on account of requests from a commercial tissue culture laboratory, outside the Pacific island region for taro germplasm and the relevant multiplication technology (Taylor, 1996). The issue of germplasm exchange that aroused regional interest and concern, has led to the consideration of emerging pressures which emphasise the need for the Pacific island countries to consider some form of intellectual property rights system.

These pressures are:

    • The need to comply with the directives of the World Trade Organisation to its members to institute a system of plant variety protection either through the use of patents or an 'effective sui generis system' by 1999;
    • Increased bioprospecting in the Pacific island region, that is being practised often without prior informed consent and mutually agreed terms between the bioprospectors and the country in possession of the desired resources; and
    • Imminent loss of commercial markets of medicinal plants such as kava that are of significance in the pharmaceutical industry.

On the issue of genebanks, the regional laboratories in the South Pacific are responsible for a wide range of crop germplasm originating from many different island countries in Polynesia, Melanesia and Micronesia. These collections were originally assembled on the principle of 'common heritage' in the absence of a defined policy regarding germplasm exchange. Today, with the galloping advances being made in deriving benefits from the applications of biotechnology, the Pacific island countries have become concerned about the possible patenting of their plant and microbial genetic resources.

What type of biotechnology development is therefore appropriate to the Caribbean and Pacific Regions? In response, the national economic and social objectives have been identified (FAO, 1992). These deal with the achievement of sustainable growth in the commercial production of quality agricultural products for domestic consumption; and the development of appropriate regional strategies that help to counteract the import-oriented markets that cripple the economies of these regions. These strategies are also of relevance to islands in the Asian and African environments. In the run-up to the new millennium, it is anticipated that all of the tissue culture laboratories in the six non-atoll PACP countries will be functioning well, producing planting material for growers, assisting in the safe importation of plants and conserving some of the region's most important genetic resources.

Conclusion

Despite their fragile economies aspects that are also characteristic of Asian and African small islands (Table 2), and their vulnerability to the vicissitudes of Nature, very few of the Caribbean and Pacific islands are encountered in the category of the least developed countries. The new millennium will bring with it the graduation of Samoa from the list.

Table 2. ABN member countries and priorities

Regions

Central Africa

Eastern and Southern Africa

West Africa

Burundi, Cameroon,
Central African Republic, Chad, Congo, Gabon, Rwanda, Zaire

Angola, Botswana, Ethiopia, Equatorial Guinea, Kenya, Lesotho, Malawi, Mozambique, Uganda, Swaziland

Benin, Burkina Faso,
Cape Verde, Cote d'Ivoire, Ghana, Guinea Conakry, Liberia, Mali, Mauritania, Niger, Nigeria, Senegal, Sierra Leone

Priorities areas

Animal breeding, endemic diseases, forest resources, insect pests, medicinal plants, microbiology, nutritional problems, ornamental plants breeding, water resources

Globalisation has embraced the world of islands in Asia, Africa, Europe, the Caribbean and the pacific regions. As a result, and despite the geographic and economic limitations that slow down the pace of technological advancement, several island nations have embarked upon the path of diversifying and modernising their reliance on traditional agricultural and Nature-given biotech resources. Amongst the various options, virtually all island countries, inclusive of Cyprus and Malta, have adopted high-tech biotechnology, genetic engineering, and gene-rich micro-organisms as strategic mechanisms in ensuring self-reliant development and economic stability for the year 2000 and beyond.

References

Clancy, J.S. and Hulscher, W.S. (1994). The possibilities of biogas production in developing countries. Special programme for biotechnology development co-operation. Directorate-General for International Co-operation, Ministry of Foreign Affairs, The Hague, Netherlands.

DaSilva, E.J. (1998). Biotechnology: Developing Countries and Globalisation. World Journal of Microbiology and Biotechnology 14:463-486.

Jackson G.V.H. (1994). Taro and Yam resources in the Pacific and Asia, Report for ACIAR and IPGRI, Anutech Pty., Ltd

O'Garro, L. (1997). Prospects for Control of Bacterial Spot of Pepper and Tomato in the Anglophone Eastern Caribbean: A Research Model for the Application of Biotechnology in Small Developing Countries, UNESCO, Port of Spain, pp. 99

FAO, (1992). Current Status and Future Prospects of Modern Biotechnologies in Latin America and the Caribbean. Executive Summary 22nd Regional Conference for Latin America and the Caribbean, 28 September - 2 October, Montevideo, Uruguay. Document LARC/92/4 - W/Z9328/c.

Taylor M. (1996) Intellectual property rights and plant germplasm in the South Pacific region. PRAP Working Paper No. 96-3. Suva: Pacific Regional Agricultural Programme (PRAP). Project 7. Provision of tissue Culture Services for the Region.

Supported by UNESCO / MIRCEN network
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