Figure 5. Sugarcane transformation by Agrobacterium tumefaciens. A-F. (A) Selection of PTT-resistant calli on selective medium, supplemented with 4 mg/L of PPT. (B) Histochemical GUS-activity assay in a shoot-developing PPT resistant callus transformed by A. tumefaciens bearing the pGT GUSBAR plasmid; in the top, non-transformed shoot-developing callus. (C) Regeneration of transformants from PPT-resistant calli on selective medium, supplemented with 4 mg/L of PPT (bottom), compared with necrosed non-transformed calli on the same medium (top). (D) Whole plant GUS expression assay in two transformed individuals (after regeneration). (E) Sugarcane plants under greenhouse conditions two weeks after the application of the herbicide. Plants were sprayed with BASTA at 2.5 g/L; non-transgenic plants turned brown and died. (F) Sugarcane plants under field conditions, The lanes between the field way are not transformed and died after application of the herbicide.