Molecular Biology and Genetics
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 1, Issue of January 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received April 18, 2006 / Accepted October 5, 2006
DOI: 10.2225/vol10-issue1-fulltext-10  
TECHNICAL NOTE

Ethanol mediated enhancement in bacterial transformation

Arun Dev Sharma*
 Department of Biotechnology
Lyallpur Khalsa College
Jalandhar, Punjab, India
Tel: 91 0181 2241466 68
Fax: 91 0181 2241465
E-mail: arundevsharma@rediffmail.com

Jaspreet Singh
 Department of Biotechnology
Lyallpur Khalsa College
Jalandhar, Punjab, India

Prabhjot Kaur Gill
Lovely Institute of Higher Studies
Phagwara, Near Chearu Bridge
Dist- Kapurthala, Punjab, India 

*Correponding author 

Financial support: Management Lyallpur Khalsa College.

Keywords: bacteria, ethanol, transformation.

Abbreviations:

LB: Luria Broth
Abstract
Full Text

In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
Supported by UNESCO / MIRCEN network