Abstract - Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by Amplified Fragment Length Polymorphism markers

Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by Amplified Fragment Length Polymorphism markers

Ghulam M. Ali*
Agriculture Biotechnology Program
National Agriculture Research Center
Park Road, Islamabad, Pakistan
Tel: 92 51 9255217
Fax: 92 51 9255034
E-mail: drgmali@yahoo.ca

Sirato Yasumoto
Laboratory of New Crops Breeding
National Institute of Crop Sciences
National Agriculture Research Organization
Kannondai 2-1-18 Tsukuba, Ibaraki 305-8666, Japan
Tel: 81 29 838 8393
Fax: 81 29 838 8475
E-mail: katsuta@affrc.go.jp

Masumi Seki-Katsuta
Laboratory of New Crops Breeding
National Institute of Crop Sciences
National Agriculture Research Organization
Kannondai 2-1-18 Tsukuba, Ibaraki 305-8666, Japan
Tel: 81 29 838 8393
Fax: 81 29 838 8475
E-mail: katsuta@affrc.go.jp

*Corresponding author

Financial support: This work was performed under the fellowship for G.M. Ali. Authors are grateful to Japan Society for Promotion of Science and Natural Science and Engineering Research Council Canada for this award and financial support.

Keywords: AFLP, genetic diversity, geographical origin, morphology, sesame.

Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia and Africa. To determine the level of genetic diversity in relation to geographical origins and morphological characteristics, a total of 96 accessions have been collected from different parts of the world and were analyzed using AFLP techniques. Twenty-one primer pairs generated a total of 445 bands and among them 157 (35%) were polymorphic. Using UPGMA clustering analysis method based on the similarity coefficient, accessions were separated into two major groups. The first group mostly consists of Eastern Asian origin and another group consists of South Asian origin. Sub-clusters separated the accessions and form distinct diversity among groups. Considering the relatednessof accessions, geographical origin and their morphological characteristics are reflected to the similarity of AFLP pattern.