Process Biotechnology

Biotechnology Industry
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 1, Issue of January 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received November 14, 2005 / Accepted July 25, 2006
DOI: 10.2225/vol10-issue1-fulltext-3  
RESEARCH ARTICLE

Supercritical fluid CO2 extraction of essential oil from Marchantia convoluta: global yields and extract chemical composition

Jian Bo Xiao#
Jiangsu Provincial Key Laboratory
of Coastal Wetland Bio-resources and Environmental Protection
Yancheng Normal College
Yancheng 224002, P. R. China
Tel: 86 515 8298130
E-mail: jianbo_xiai@yahoo.com.cn

Jing Wen Chen*
Yancheng Institute of Technology
Yancheng 224003, P. R. China
Tel: 86 515 8168323
E-mail: chenjingwen2005@yahoo.com.cn

Ming Xu§
Research Institute for Molecular Pharmacology and Therapeutics
Central South University
Changsha 410000, P. R. China
Tel: 86 731 8830833
E-mail: newfourtharmy@163.com

*Corresponding author

Financial support: Jiangsu Provincial Key Laboratory of Coastal Wetland Bio-resources and Environmental Protection (JLCBE06002).

Keywords: GC-MS, Marchantia convoluta, supercritical fluid extraction, yields.

Present address: #College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha 410083, PR China. ¡ìDepartment of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York 10021, USA.

Abbreviations:

SFE: Supercritical fluid extraction
GC-MS: Gas chromatography-mass spectrometry
Abstract
Full Text

The essential oil of Marchantia convoluta was obtained by supercritical (carbon dioxide) extraction using methanol as a modifier. Global yields were determined according to the orthogonal design. The effects of different parameters, such as pressure, temperature, modifier volume and extraction time, on the supercritical fluid extraction (SFE) of essential oil from M. convoluta were investigated. Maximum global yields were obtained using the following conditions: extraction temperature, 35ºC; dynamic time, 35 min; pressure, 15 Mpa and modifier volume, 40 mL. The essential oil extract was analyzed by capillary gas chromatography with mass spectrometric detector (GC-MS). The compounds were identified according to their retention indices and mass spectra (EI, 70 eV). The results from GC-MS and literature were compared.
Supported by UNESCO / MIRCEN network