Environmental Biotechnology

Biofilms

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 1, Issue of January 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received May 8, 2006 / Accepted August 14, 2006
DOI: 10.2225/vol10-issue1-fulltext-4  
SHORT COMMUNICATION

Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution

Carlucio R. Alves*
Environmental Biotechnology Group
State University of Ceará
60.640-020 Fortaleza, Ceará, Brazil
Tel: 55 85 3101 9822
Fax: 55 85 3299 2500
E-mail: alvescr@yahoo.com

Maria Gardenny R. Pimenta
Environmental Biotechnology Group
State University of Ceará
60.640-020 Fortaleza, Ceará, Brazil
Tel: 55 85 3101 9822
Fax: 55 85 3299 2500
E-mail: gardenny@yahoo.com.br

Regine H.S.F. Vieira
Science Institute - Labomar
Federal University of Ceará
60.165-081 Fortaleza, Ceará, Brazil
Tel: 55 85 3101 9822
Fax: 55 85 3299 2500
E-mail: regine@labomar.ufc.br

Roselayne F. Furtado
Environmental Biotechnology Group
State University of Ceará
60.640-020 Fortaleza, Ceará, Brazil
Tel: 55 85 3101 9822
Fax: 55 85 3299 2500
E-mail: roselayneff@hotmail.com

Maria Izabel F. Guedes
Environmental Biotechnology Group
State University of Ceará
60.640-020 Fortaleza, Ceará, Brazil
Tel: 55 85 3101 9822
Fax: 55 85 3299 2500
E-mail: florinfg@terra.com.br

Rui C.B. Silva
Environmental Biotechnology Group
State University of Ceará
60.640-020 Fortaleza, Ceará, Brazil
Tel: 55 85 3101 9822
Fax: 55 85 3299 2500
E-mail: rcbs@uece.br

Odilio B.G. Assis
Embrapa Agricultural Instrumentation Center
Caixa Postal 741, 13560-970
São Carlos, SP, Brazil
Tel: 55 16 2742477
Fax: 55 16 2725958
E-mail: odilio@cnpdia.embrapa.br

*Corresponding author

Financial support: This project was financed by a grant from FUNCAP, FAPESP and CNPq.

Keywords: Escherichia coli, lysozyme, self-assembly.

Abstract
Full Text

The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameter.

Supported by UNESCO / MIRCEN network