| Electronic Journal of Biotechnology ISSN: 0717-3458 |
Vol.
10 No. 1, Issue of January 15, 2007 |
| © 2007 by Pontificia Universidad Católica
de Valparaíso -- Chile |
Received May 19, 2006 / Accepted August 21, 2006 |
| DOI: 10.2225/vol10-issue1-fulltext-9 |
|
Influence of 2,4-D and BAP
on callus growth and the subsequent regeneration of somatic embryos
in long-term cultures of Pelargonium x domesticum cv.
Madame Layal
Klaus-Thomas
Haensch
Institute
of Vegetable
and Ornamental Crops Großbeeren/Erfurt e.V.
Department Plant Propagation
Kühnhäuser Str. 101, D-99189 Erfurt-Kühnhausen
Germany
Tel: 49 0 36 201 785 224
Fax: 49 0 36 201 785 250
E-mail:
haensch@erfurt.igzev.de
Website: http://www.igzev.de
Financial support: This investigation
was undertaken with the support of the Ministries of Agriculture of
the Federal Republic of Germany and the States of Brandenburg and
Thüringen.
Keywords: auxin, cytokinin,
pelargonium, propagation, somatic embryogenesis.
| Abbreviations: |
2,4 D: 2,4 dichlorophenoxyacetic
acid
BAP: 6 benzylaminopurine
|
This
long-term study demonstrates for the first time that it is possible
to propagate embryogenic cultures in pelargoniums and to subsequently
initiate the differentiation of embryos using the cultivar Madame
Layal (Pelargonium x domesticum). Propagation of callus
was only possible with combinations of 2,4-dichlorophenoxyacetic acid
(2,4-D) and 6-benzylaminopurine (BAP), which gave rise to embryos
from the primary culture stage on. However, the propagation of cells,
as well as the differentiation of embryos, was inhibited by a continuous
application of these growth regulators. For this reason, a long period
on medium lacking growth regulators was necessary before the differentiation
of embryos occurred again. The consequences for improving the propagation
of embryogenic cultures in pelargoniums are discussed.
|
