Table 1. Composition of media used at various stages of micropropagation of NR banana. |
Medium |
Composition with growth regulators in mg l-1 |
Stages |
M1 |
MS* + BAP (0.2) + Sucrose (3%) + GelriteŽ (0.2%) |
Meristem culture |
M2 |
MS*
+ BAP (2) + Kn (1) + AS (80) + |
Shoot multiplication |
M3-A |
MS* + NAA (0.5) + Sucrose (2.5%) (Liquid medium) |
Elongation and rooting |
M3-B |
MS*
+ NAA (0.5) + Sucrose (2.5%) + |
Semi-hardening
and further |
M4 |
˝ MS + Agar (1.6%) |
Hardening |
* Modified MS where the concentration of ammonium nitrate was reduced to 75% of original MS medium (Murashige and Skoog, 1962). |
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