Table 1. Composition of media used at various stages of micropropagation of NR banana.


Medium

Composition with growth regulators in mg l-1

Stages

M1

MS* + BAP (0.2) + Sucrose (3%) + GelriteŽ (0.2%)

Meristem culture

M2

MS* + BAP (2) + Kn (1) + AS (80) +
Sucrose (2.5%) + GelriteŽ (0.25%)

Shoot multiplication

M3-A

MS* + NAA (0.5) + Sucrose (2.5%) (Liquid medium)

Elongation and rooting

M3-B

MS* + NAA (0.5) + Sucrose (2.5%) +
Agar (0.8%) (Solid medium)

Semi-hardening and further
elongation and rooting

M4

˝ MS + Agar (1.6%)

Hardening

* Modified MS where the concentration of ammonium nitrate was reduced to 75% of original MS medium (Murashige and Skoog, 1962).


Supported by UNESCO / MIRCEN network