Table 2. List of primers, their sequences and size of the amplified fragments generated by 30 RAPD (Sl. No. 1-30) and 5 ISSR (Sl. No. 31-36) primers.

Sl.
No.

Primer

Primer sequence
(5’ – 3’)

G + C
Content
(%)

Mg Cl₂
Concentration
(mM)

Number of
scorable bands

Size range
(bp)

1

OPA-04

AATCGGGCTG

60

2

12

300-3000

2

OPA-09

GGGTAACGCC

70

0

8

400-1800

3

OPA-11

CAATCGCCGT

60

2

18

300-3100

4

OPA-14

CTCGTGCTGG

70

0

13

200-3000

5

OPA-20

GTTGCGATCC

60

1

14

200-2600

6

OPC-01

TTCGAGCCAG

60

1

12

280-1800

7

OPC-02

GTGAGGCGTC

70

2

12

300-2800

8

OPC-04

CCGCATCTAC

60

0

15

220-2000

9

OPC-05

GATGACCGCC

70

0

14

320-1900

10

OPC-07

GTCCCGACGA

70

0

16

300-2600

11

OPC-08

TGGACCGGTG

70

1

14

290-3100

12

OPC-09

CTCACCGTCC

70

1

10

480-1800

13

OPC-13

AAGCCTCGTC

60

0

8

480-1800

14

OPD-07

TTGGCACGGG

70

1

13

380-2500

15

OPD-08

GTGTGCCCCA

70

0

7

290-2400

16

OPD-16

AGGGCGTAAG

60

1

14

400-2000

17

OPJ-07

CCTCTCGACA

60

1

11

300-2000

18

OPJ-08

CATACCGTGG

60

0

6

400-3000

19

OPJ-09

TGAGCCTCAC

60

2

8

400-2000

20

OPL-14

TCGTGCGGGT

70

1

8

1031-2400

21

OPM-12

CACAGACACC

60

0

12

300-2600

22

OPM-16

GTAACCAGCC

60

2

18

400-2000

23

OPM-20

AGGTCTTGGG

60

1

22

320-2000

24

OPM-18

CACCATCCGT

60

0

7

400-2000

25

OPN-03

GGTACTCCCC

70

0

18

300-2600

26

OPN-04

GACCGACCCA

70

0

12

400-2800

27

OPN-06

GAGACGCACA

60

2

11

340-2000

28

OPN-09

TGCCGGCTTG

70

1

18

240-3000

29

OPN-10

ACAACTGGGG

60

1

19

240-3000

30

OPN-12

CACAGACACC

60

0

7

240-2400

Total

377

31

UBC-811

(GA)₈C

-

0

7

250-2500

32

UBC-817

(CA)₈A

-

1

8

400-2800

33

UBC-820

(GT)₈T

-

0

11

200 - 2000

34

UBC-826

(AC)₈C

-

0

8

400- 2000

35

UBC-834

(AG)₈YT

-

1

13

1031 – 3000

Total

47