Figure 1. In vitro propagation
of O. prostrata through direct organogenesis.
(a) Shoots from
internode explants on MS medium with 8.87 µM BA and 2.46 µM IBA.
(b) Shoots from
leaf explants on MS medium with 8.87 µM BA and 2.46 µM IBA.
(c) Shoot multiplication
on the above medium during subculture.
(d) Shoot origin
from sub-epidermal cells of leaf explants (bar = 0.1
mm).
(e) Shoot origin
from sub-epidermal cells of internode explants (bar = 0.1
mm).
(f) Rooted shoot
on half-strength MS medium with 10.74 mM NAA and 2.32 mM Kn.
(g) Established
plant (after 45 days). |