Table 1 - Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant

Table 1. Direct organogenesis from internode and proximal leaf explants of O. prostrata on MS media with various plant growth regulators. Data represents the mean of 20 replicates. Mean values followed by the same letters within columns are not significantly different at the 5% level. Culture duration was 40 days.

Plant Growth regulators (µM)				Percentage of explants forming shoots		Number of shoots per explant
BA	Kn	NAA	IBA	Leaf	Internode	Leaf	Internode
0	0	0	0	80	90	2.9^m	4.0^l
2.22				80	85	8.4^k	12.3^j
4.44				95	100	13.8^j	25.9^g
6.66				100	100	21.4^g	35.1^e
8.87				100	100	38.0^d	51.5^d
13.3				100	100	30.4^e	36.5^e
17.7				100	100	25.0^f	29.3^f
22.2				100	100	13.4^j	17.6^h
	2.32			90	95	4.1^l	6.2^k
	4.65			100	100	19.1^g	27.0^fg
	6.97			100	100	16.2ⁱ	18.6^h
	9.29			100	100	7.5^k	14.7ⁱ
8.87	0.46			100	100	24.5^f	29.5^f
8.87	2.32			100	100	21.3^g	24.3^g
8.87	4.65			100	100	16.1ⁱ	21.5^h
8.87		0.54		100	100	29.3^e	35.6^e
8.87		2.69		100	100	24.5^f	28.8^f
8.87		5.37		100	100	19.2^g	24.1^g
8.87			0.49	100	100	47.5^b	61.0^b
8.87			2.46	100	100	76.0^a	90.8^a
8.87			4.90	100	100	40.7^c	56.1^c