Molecular Biology and Genetics

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 2, Issue of April 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received August 16, 2006 / Accepted December 20, 2006
DOI: 10.2225/vol10-issue2-fulltext-14
SHORT COMMUNICATION

Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter

Rob Lavigne
Division of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-3001, Belgium
Tel: 32 0 16 32 96 70
Fax: 32 0 16 32 19 65
E-mail: rob.lavigne@biw.kuleuven.be

Guido Volckaert*
Division of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-3001, Belgium
Tel: 32 0 16 32 96 67
Fax: 32 0 16 32 19 65
E-mail: guido.volckaert@biw.kuleuven.be

*Corresponding author

Financial support: RL holds a postdoctoral fellowship from the FWO (Fonds voor Wetenschappelijk Onderzoek), Vlaanderen.

Keywords: cognate gene plasmid, functional complementation, homolog, Saccharomyces cerevisiae, yeast.

Abbreviations:

DRR: downstream regulatory region
EUROFAN: European Functional Analysis Network
EUROSCARF: EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis
ORF: Open Reading Frame
URR: upstream regulatory region

Abstract   Full Text

A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expression.

Supported by UNESCO / MIRCEN network