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Molecular
Biology and Genetics |
Electronic Journal of Biotechnology ISSN: 0717-3458 |
Vol.
10 No. 2, Issue of April 15, 2007 |
© 2007 by Pontificia Universidad Católica
de Valparaíso -- Chile |
Received August
16, 2006 / Accepted December 20, 2006 |
DOI: 10.2225/vol10-issue2-fulltext-14 |
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Functional
complementation in Saccharomyces cerevisiae under control of
the natural yeast promoter
Rob Lavigne
Division
of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-3001, Belgium
Tel: 32 0 16 32 96 70
Fax: 32 0 16 32 19 65
E-mail: rob.lavigne@biw.kuleuven.be
Guido Volckaert*
Division
of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-3001, Belgium
Tel: 32 0 16 32 96 67
Fax: 32 0 16 32 19 65
E-mail: guido.volckaert@biw.kuleuven.be
*Corresponding
author
Financial support:
RL holds
a postdoctoral fellowship from the FWO (Fonds voor Wetenschappelijk
Onderzoek), Vlaanderen.
Keywords:
cognate gene plasmid, functional complementation, homolog, Saccharomyces
cerevisiae, yeast.
Abbreviations: |
DRR: downstream regulatory region
EUROFAN: European Functional Analysis Network
EUROSCARF: EUROpean Saccharomyces Cerevisiae ARchive
for Functional analysis
ORF: Open Reading
Frame
URR: upstream regulatory region
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A
novel approach for functional complementation of foreign genes in
Saccharomyces cerevisiae is presented. This approach is based
on the use of the widely available cognate gene plasmids (e.g.
pRS416) of the European Functional Analysis Network (EUROFAN). The
functional complementation of the human homolog of YOR159c (SME1
gene) shown here is the first demonstration of complementation using
the original yeast promoter, theoretically offering a more natural
regulation of protein expression.
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