Microbial Biotechnology

Process Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 2, Issue of January 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received December 28, 2005 / Accepted October 31, 2006
DOI: 10.2225/vol10-issue2-fulltext-8
RESEARCH ARTICLE

Influence of medium constituents on the biosynthesis of cephalosporin-C

Vinod Kumar Nigam*
 Birla Institute of Scientific Research Statue Circle
Jaipur, Rajasthan 302001, India
Tel: 911412385283
Fax: 911412385121
E-mail: nigam_vinod@yahoo.com 

Ruchi Verma
 Birla Institute of Scientific Research Statue Circle
Jaipur, Rajasthan 302001, India
Tel: 911412385283
Fax: 911412385121
E-mail: verma_ruchi03@rediffmail.com 

Abhishek Kumar
 Birla Institute of Scientific Research Statue Circle
Jaipur, Rajasthan 302001, India
Tel: 911412385283
Fax: 911412385121

Subir Kundu
 School of Biochemical Engineering
Institute of Technology
Banaras Hindu University
Varanasi, Uttar Pradesh 221005
India
Tel: 915422367829
Fax: 915422368693
E-mail: subirbhu@gmail.com

Purnendu Ghosh
 Birla Institute of Scientific Research Statue Circle
Jaipur, Rajasthan 302001, India
Tel: 911412385283
Fax: 911412385121
E-mail: pghosh9@yahoo.com

*Corresponding author

Financial support: Department of Science and Technology, Government of India for carrying out this work.

Keywords: Acremonium chrysogenum, batch fermentation, cephalosporin-C, methionine, stirred tank bioreactor.

Abbreviations:

CPC: cephalosporin-C
STBR: stirred tank bioreactor
Abstract
Full Text

Acremonium chrysogenum NCIM 1069 was used for the biosynthesis of cephalosporin-C (CPC) in batch mode of cultivation. The effect of different medium constituents for better yield of CPC was thoroughly investigated. From the results of the fermentation, it was found that ammonium sulphate as inorganic nitrogen source and methionine at the concentration of 0.4% are most suitable for higher yield of antibiotic. The variation in the C/N ratio on the biosynthesis of CPC showed that a C/N ratio of 8.0 is most suitable for maximum production of CPC.
Supported by UNESCO / MIRCEN network