Figure 2. Comparison of luciferase expression from the SV40 promoter of a conventionally cloned plasmid and plasmids prepared using Cre-loxP based cloning. 4 individual Cre-loxP cloned plasmids and a conventionally cloned plasmid with the SV40 promoter regulating expression of luicferase were transiently transfected into 2 cell lines and luciferase activities were compared. The data shown is the mean of duplicates with S.E.M.