Figure 4. Expression of reporter genes from Cre-loxP cloned promoters.
(a) GLC 16 cells were transiently transfected with EGFP and DsRed Cre-loxP generated plasmids as indicated on the figure and photographed after 24 hrs. The left frames are in phase contrast and the right frames with fluorescence using 10 x magnification.
(b) GLC 16 cells were transfected with the β-galactosidase gene regulated by the SV40 promoter cloned using the Cre-recombinase system and stained with X-gal after 48 hrs.