Table 1. Effect of fermentation temperature and extended incubation in the stationary growth phase (5 hrs) on biomass levels of Lactobacillus rhamnosus RW-9595M, medium CRV and the subsequent removal of cells by centrifugation.

Fermentation

Fermentation
time (hr)

Population
(CFU/ml)

Cells per
chain¹

CRV

OD

Temperature

Growth phase

Before
CF²

After CF
4000 g

After CF
10000 g

37ºC

Beginning stationary

14.2 (0.4)

9.3 (0.9) x 10⁹

1.3 (0.1)

4.81 (0.29)

11.1 (0.1)

5.7 (0.1)

5.8 (0.1)

42ºC

Beginning stationary

12.8 (0.3)

7.2 (1.3) x 10⁹

1.7 (0.1)

2.66 (0.27)

11.8 (0.3)

5.9 (0.1)

1.7 (1.1)

42ºC

5 hrs in stationary

17.8 (0.3)

5.7 (0.6) x 10⁹

1.8 (0.2)

2.97 (0.32)

11.4 (0.3)

5.8 (0.1)

2.4 (1.0)

44ºC

Beginning stationary

11.5 (0.6)

6.3 (1.1) x 10⁹

1.5 (0.3)

2.20 (0.06)

12.0 (0.2)

5.8 (0.1)

0.1 (0.1)

44ºC

5 hrs in stationary

16.5 (0.6)

5.4 (0.6) x 10⁹

2.0 (0.4)

2.25 (0.04)

11.7 (0.3)

5.7 (0.1)

0.1 (0.1)