Figure 1. Method for functional complementation under yeast promoter control. Amplification of the Upstream and Downstream Regulatory Regions (URR and DRR) is followed by their ligation. This ligation product is amplified using the yORF-F and yORF-R primers and inserted in pRS416 by BamHI ligation. The human Open reading frame (BC002639) can subsequently be cloned in the Bg/II site between the regulatory regions. Thus, extraneous sequences are limited to 6 bp in the downstream region and 2 codons at the 5’ of the ORF.