Figure 6. Culture for adaptation of CHOZMD cells on microcarrier Pronectin F for production of the recombinant disintegrin in serum free medium (CHO-SFMII). The culture was initiated with medium DMEM + 10% v/v of serum. The arrows indicate the times of medium replacement with increasing concentrations (% v/v) of CHO-SFMII: M1 (5%), M2 (12.5%), M3 (15%), M4 (20%), M5 (25%), M6 (30%), M7 (40%), M8 (50%), M9 (50%) and M10 (100%).