Table 2. Values of the adhesion parameters of CHOZMD cells on the four microcarriers calculated by nonlinear fitting of two adhesion models to experimental data.


Microcarrier

Fitting results with the hyperbolic model
Equation:

Parameters:
Naf (cells.mL-1) and Ka(h)

Fitting results with Ng et al. (1996)
first order model
Equation:
Na=Naf[1- exp(-kat)]
Parameters:
Naf (cells.mL-1) and ka(h-1)

Parameter ± SE

R

Parameter ± SE

R

 Cytodex 1

Naf = 303,726 ± 9,863
Ka = 0.909 ± 0.114

0.991

Naf = 257,682 ± 8,060
ka = 0.918 ± 0.104

0.992

 Cultispher S

Naf = 280,350 ±105
Ka = 0.278 ± 0.001

1.000

Naf = 265,988 ± 1,170
ka= 1.290 ± 0.049

1.000

 Pronectin F

Naf = 330,585 ± 12,022
Ka = 5.125 ± 0.340

0.984

Naf = 224,757 ± 8,354
ka = 0.261 ± 0.018

0.983

 Immobasil FS

Naf = 250,763 ± 2,535
Ka = 0.846 ± 0.038

0.997

Naf = 213,531 ± 3,558
ka = 0.915 ± 0.068

0.991

SE: Standard error.
R: Correlation coefficient.


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