Table 1. Effect of Tween 80, Tween 20 and Triton X-100, chelator and inhibitors on crude tannase activity.

Additives

Concentration
(%(v/v))

Relative activity
(%)

Control

-

100 ± 0.64

Tween 80

0.25

85 ± 1.83

0.5

77 ± 1.53

1.0

70 ± 1.84

Tween 20

0.25

81 ± 2.12

0.5

75 ± 2.44

1.0

66 ± 0.91

Triton X-100

0.25

96 ± 0.31

0.5

93 ± 0.61

1.0

87 ± 0.62

Control

-

100 ± 2.67

EDTA

1 mM

62 ± 3.59

Sodium bisulphite

1 mM

75 ± 4.40

Iodoacetamide

1 mM

96 ± 1.26

2-mercaptoethanol

1 mM

59 ± 3.14

4-aminobenzoic acid

1 mM

75 ± 3.45

Sodium azide

1 mM

64 ± 3.14

N- bromosuccinimide

1 mM

71 ± 5.60

Cysteine

1 mM

52 ± 3.14