Plant Biotechnology

Molecular Biology and Genetics
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 3, Issue of July 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received January 9, 2006 / Accepted March 5 , 2007
DOI: 10.2225/vol10-issue3-fulltext-10
TECHNICAL NOTE

Isolation of simple sequence repeats from groundnut

Chuan Tang Wang*
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 87626662
Fax: 86 532 87626832
E-mail: chinapeanut@126.com

Xin Dao Yang
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 88433679
Fax: 86 532 87626832
E-mail: smdkrdc@126.com

Dian Xu Chen
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 87626723
Fax: 86 532 87626832
E-mail: Chenzhao126@sina.com

Shan Lin Yu
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 87626830
Fax: 86 532 87626832
E-mail: rice407@sohu.com

Guang Zhen Liu
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 88411241
Fax: 86 532 88411241
E-mail: wangzhuo1995@126.com

Yue Yi Tang
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 87626662
Fax: 86 532 87626832
E-mail: tangyueyi0597@sina.com

Jian Zhi Xu
Shandong Peanut Research Institute
Qingdao 266100
P R China
Tel: 86 532 88411707
Fax: 86 532 87626832
E-mail: Tsingtao2008@126.com
 
*Corresponding author

Financial support: The research was supported in part by grants from China Natural Science Foundation (Grant No. 30300224), China Ministry of Science and Technology (Grant No. 2002CCC03200, Grant No. 2006AA10A114), and New and High Technology Innovation Foundation of Shandong Academy of Agricultural Sciences (Grant No. 2006 YCX013).

Keywords: groundnut, isolation, simple sequence repeat.

Abbreviations:

AFLP: amplified fragment length polymorphism
MW: molecular weight
PCR: polymerase chain reaction
SDS: sodium dodecyl sulfate
SSR: simple sequence repeat
Abstract
Full Text

SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts.
Supported by UNESCO / MIRCEN network