Molecular description and similarity relationships among native germplasm potatoes (Solanum tuberosum ssp. tuberosum L.) using morphological data and AFLP markers
Jaime Solano Solis
Daniza Morales Ulloa
Leonardo Anabalón Rodríguez*
Financial support: The present work has been done in the framework of a research programme DIPUCT (Grant DIPUCT 2004-2-02) of the Universidad Católica de Temuco.
Keywords: cluster analysis, molecular markers, Solanum tuberosum ssp. tuberosum.
Chile is considered to be a sub-center of origin for the cultivated potato, with native and introduced genetic material coexisting in the country. Thus, the different varieties present in Chiloe Island are characterized by a rich diversity of forms, sizes, colours and phenological characteristics. In the present work, the level of polymorphism and the genetic relationship were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique and twenty-seven morphological characters. Twenty varieties of potatoes from the Chiloe Island were analyzed. The commercial variety Desirée and one specie from the Etuberosa series, Solanum fernandezianum, collected in the Juan Fernandez Island were included as controls. A similarity tree-diagram was made, based on all the AFLP bands generated in the range between 65 and 290 base pairs. With these tools, it was possible to identify molecular differences and similarities that might be associated with important morphological traits such as the predominant forms of the tuber, flower colour and resistance to disease.