Molecular Biology and Genetics

Plant Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 3, Issue of July 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received April 10, 2006 / Accepted March 23, 2007
DOI: 10.2225/vol10-issue3-fulltext-14
RESEARCH ARTICLE

Molecular description and similarity relationships among native germplasm potatoes (Solanum tuberosum ssp. tuberosum L.) using morphological data and AFLP markers

Jaime Solano Solis
Laboratorio de Biotecnología y Mejoramiento Vegetal
Escuela de Agronomía
Universidad Católica de Temuco
Longitudinal Norte s/n Temuco, Chile
Tel: 56 45 205527
Fax: 56 45 205540
E-mail: jsolano@uct.cl

Daniza Morales Ulloa
Laboratorio de Biotecnología y Mejoramiento Vegetal
Escuela de Agronomía
Universidad Católica de Temuco
Longitudinal Norte s/n Temuco, Chile
Tel: 56 45 205527
Fax: 56 45 205540

Leonardo Anabalón Rodríguez*
Laboratorio de Biotecnología y Mejoramiento Vegetal
Escuela de Agronomía
Universidad Católica de Temuco
Longitudinal Norte s/n Temuco, Chile
Tel: 56 45 205527
Fax: 56 45 205540
E-mail: lanabalo@uct.cl

*Corresponding author

Financial support: The present work has been done in the framework of a research programme DIPUCT (Grant DIPUCT 2004-2-02) of the Universidad Católica de Temuco.

Keywords: cluster analysis, molecular markers, Solanum tuberosum ssp. tuberosum.

Abbreviations:

AFLP: amplified fragment length polymorphism
PCR: polimerase chain reaction

Abstract
Full Text

Chile is considered to be a sub-center of origin for the cultivated potato, with native and introduced genetic material coexisting in the country. Thus, the different varieties present in Chiloe Island are characterized by a rich diversity of forms, sizes, colours and phenological characteristics. In the present work, the level of polymorphism and the genetic relationship were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique and twenty-seven morphological characters. Twenty varieties of potatoes from the Chiloe Island were analyzed. The commercial variety Desirée and one specie from the Etuberosa series, Solanum fernandezianum, collected in the Juan Fernandez Island were included as controls. A similarity tree-diagram was made, based on all the AFLP bands generated in the range between 65 and 290 base pairs. With these tools, it was possible to identify molecular differences and similarities that might be associated with important morphological traits such as the predominant forms of the tuber, flower colour and resistance to disease.

Supported by UNESCO / MIRCEN network