Plant Biotechnology

Molecular Biology and Genetics
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 3, Issue of July 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received August 28, 2006 / Accepted January 19, 2007
DOI: 10.2225/vol10-issue3-fulltext-5
TECHNICAL NOTE

A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot

Vishal P. Deshmukh
 Department of Botany
Sant Gadge Baba Amravati University
Amravati 444602, India
Tel: 091 721 2662207
Fax: 091 721 2660949
E-mail: vishalpdeshmukh@yahoo.com

Prashant V. Thakare*
Department of Biotechnology
Sant Gadge Baba Amravati University
Amravati 444602, India
Tel: 091 721 2662249
Fax: 091 721 2660949
E-mail: prashantthakare123@rediffmail.com

Uddhav S. Chaudhari
Department of Botany
Sant Gadge Baba Amravati University
Amravati 444602, India
Tel: 091 721 2662207
Fax: 091 721 2660949
E-mail: druschoudhary@yahoo.co.in

Prashant A. Gawande
 Department of Botany
Sant Gadge Baba Amravati University
Amravati 444602, India
Tel: 091 721 2662207
Fax: 091 721 2660949
E-mail: prashantagawande@india.com

*Corresponding author

Financial support: Late Mukand Narhari Kale Research Scholarship Sant Gadge Baba Amravati University, Amravati.

Keywords: Ayurveda, DNA extraction, medicinal plant, Terminalia species.

Abbreviations:

PCR: polymerase chain reaction
RAPD: random amplification of polymorphic DNA
Abstract
Full Text

Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA, using in house prepared reagents.
Supported by UNESCO / MIRCEN network