Table 2. Oligonucleotide primers employed in RAPD analysis of Cratylia argentea, their sequence, number of polymorphic (P) and monomorphic (M) bands obtained, percentage of polymorphic bands (% PB), and discriminatory power (DL). |
Primer code |
Sequence |
Number of bands |
DL |
|||
(including C. mollis) |
(only C. argentea) |
|||||
P |
M |
P |
M |
|||
OPD 15 |
CATCCGTGCT |
9 |
1 |
7 |
1 |
0.903 |
OPG 12 |
CAGCTCACGA |
16 |
1 |
11 |
1 |
0.942 |
OPI 07 |
CAGCGACAAG |
16 |
1 |
12 |
2 |
0.755 |
OPJ 06 |
TCGTTCCGCA |
13 |
0 |
13 |
0 |
0.898 |
OPJ 07 |
CCTCTCGACA |
8 |
0 |
8 |
0 |
0.862 |
OPJ 12 |
GTCCCGTGGT |
6 |
1 |
5 |
1 |
0.615 |
Sum |
|
68 |
4 |
56 |
5 |
|
Total |
|
72 |
61 |
|
||
PB (%) |
|
94.4 |
91.8 |
|
|