Table 2. The quality and yields of soil samples DNA extracted by hot SDS lysis followed by bead beating and freeze thaw cycles.

Sample

Soil Source

A260/230

Yield*
(μg/g soil)

 1.

Rhizosphere

0.88 (± 0.07)

164.2 (± 5.20)

Bulk

1.00 (± 0.11)

65.5 (± 3.66)

 2.

Rhizosphere

0.77 (± 0.06)

53.5 (± 2.82)

Bulk

0.66 (± 0.03)

7.2 (± 0.30)

 3.

Rhizosphere

0.63 (± 0.03)

129.9 (± 5.14)

Bulk

0.69 (± 0.04)

112.2 (± 2.19)

*The yield of the crude DNA was calculated by assuming that an O.D. of 1 at 260 nm corresponds to 50 μg/ml for double stranded DNA (Sambrook et al. 1989).
Values in the table are mean of three replicate samples with the standard deviations in parentheses.

Supported by UNESCO / MIRCEN network