Figure 1. Kinetics of Yarrowia lipolytica growth on stearin used as the sole carbon source in flask experiments. Representation of biomass produced (X, mg/ml), substrate fat consumed (S, mg/ml) and lipid produced (L, mg/ml) when maximum concentration of cellular lipids was observed (a) and evolution of lipase produced with time (b) during growth of Yarrowia lipolytica at initial stearin concentration 10 or 20 mg/ml and variations in the initial concentration of (NH₄)₂SO₄. Culture conditions: growth on flasks, T=28 ± 1 ºC, agitation rate 180 ± 5 rpm, S₀=10 or 20 mg/ml. Kinetics conducted in at least duplicate by using different inocula.