Abstract - Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus cultured in bioreactors

Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus cultured in bioreactors

Abdullah Mohammad Shohael
Research Center for the Development of Advanced Horticultural Technology
Chungbuk National University
Cheongju 361-763, South Korea
Tel: 82 43 261 3245
Fax: 82 43 272 5369
E-mail: sohaelam@yahoo.com

Hosakatte Niranjana Murthy
Department of Botany
Karnatak University
Dharwad - 580 003, India
Tel: 91 836 221 5314
Fax: 91 836 274 7884
E-mail: nmurthy60@hotmail.com

Eun-Joo Hahn
Research Center for the Development of Advanced Horticultural Technology
Chungbuk National University
Cheongju 361-763, South Korea
Tel: 82 43 261 3245
Fax: 82 43 272 5369
E-mail: ejhahn@chungbuk.ac.kr

Kee-Yoeup Paek*
Research Center for the Development of Advanced Horticultural Technology
Chungbuk National University
Cheongju 361-763, South Korea
Tel: 82 43 261 3245
Fax: 82 43 272 5369
E-mail: paeeky@chugbuk.ac.kr

*Corresponding author

Financial support: This work is financially supported by the Ministry of Education and Human Resources Development (MOE), the Ministry of Commerce, Industry and Energy (MOCIE) and the Ministry of Labor (MOLAB), South Korea.

Keywords: eleutherosides, elicitation, methyl jasmonate, secondary metabolite, siberian ginseng, suspension culture.

This study was concentrated on the production of eleutherosides and chlorogenic acid in embryogenic suspension cultures of Eleutherococcus senticosus by exposing them to different concentrations (50-400 µM) of methyl jasmonate (MJ) during the culture period. In the bioreactor cultures, eleutheroside content increased significantly by elicitation of MJ, however, the fresh weight, dry weight and growth ratio of embryos was strongly inhibited by increasing MJ concentrations. The highest total eleutheroside (7.3 fold increment) and chlorogenic acid (3.9 fold increment) yield was obtained with 200 µM MJ treatment. There was 1.4, 3.4 and 14.9 fold increase in the eleutheroside B, E, and E1 production respectively with such elicitation treatment. These results suggest that MJ elicitation is beneficial for eleutheroside accumulation in the embryogenic cell suspension cultures.