Abstract - Multiple model approach to modelling of Escherichia coli fed-batch cultivation extracellular production of bacterial phytase

Multiple model approach to modelling of Escherichia coli fed-batch cultivation extracellular production of bacterial phytase

Olympia Roeva*
Centre of Biomedical Engineering Prof. Ivan Daskalov
Bulgarian Academy of Sciences
105 Acad. G. Bonchev Str.
Sofia 1113, Bulgaria
Tel: 359 2 979 36 48
E-mail: olympia@clbme.bas.bg

Tania Pencheva
Centre of Biomedical Engineering Prof. Ivan Daskalov
Bulgarian Academy of Sciences
105 Acad. G. Bonchev Str.
Sofia 1113, Bulgaria
Tel: 359 2 979 36 08
E-mail: tania.pencheva@clbme.bas.bg

Stoyan Tzonkov
Centre of Biomedical Engineering Prof. Ivan Daskalov
Bulgarian Academy of Sciences105 Acad. G. Bonchev Str.
Sofia 1113, Bulgaria
Tel: 359 2 979 36 48
E-mail: tzonkov@clbme.bas.bg

Michael Arndt
Institute of Technical Chemistry
University of Hannover
LeibnizUniversity of Hannover
3 Callinstrasse, 30167
Hannover, Germany
E-mail: mail@michaelarndt.net

Bernd Hitzmann
Institute of Technical Chemistry
University of Hannover
LeibnizUniversity of Hannover
3 Callinstrasse, 30167
Hannover, Germany
E-mail: hitzmann@iftc.uni-hannover.de

Sofia Kleist
Chair of Fermentation Technique
Technical Faculty
University of Bielefeld
25 Universitätsstr., 33615
Bielefeld, Germany
E-mail: sok@fermtech.techfak.uni-bielefeld.de

Gerchard Miksch
Chair of Fermentation Technique
Technical Faculty
University of Bielefeld
25 Universitätsstr., 33615
Bielefeld, Germany
E-mail: gmi@fermtech.techfak.uni-bielefeld.de

Karl Friehs
Chair of Fermentation Technique
Technical Faculty
University of Bielefeld
25 Universitätsstr., 33615
Bielefeld, Germany
E-mail: kfr@fermtech.techfak.uni-bielefeld.de

Erwin Flaschel
Chair of Fermentation Technique
Technical Faculty
University of Bielefeld
25 Universitätsstr., 33615
Bielefeld, Germany
E-mail: efl@fermtech.techfak.uni-bielefeld.de

*Corresponding author

Financial support: This work is partially supported from the National Science Fund Project No MI - 1505/2005.

Keywords: genetic algorithms, modelling, multiple model approach.

The paper presents the implementation of multiple model approach to modelling of Escherichia coli BL21(DE3)pPhyt109 fed-batch cultivation processes for an extracellular production of bacterial phytase. Due to the complex metabolic pathways of microorganisms, the accurate modelling of bioprocesses is rather difficult. Multiple model approach is an alternative concept which helps in modelling and control of complex processes. The main idea is the development of a model based on simple submodels for the purposes of further high quality process control. The presented simulations of E. coli fed-batch cultivation show how the process could be divided into different functional states and how the model parameters could be obtained easily using genetic algorithms. The obtained results and model verification demonstrate the effectiveness of the applied concept of multiple model approach and of the proposed identification scheme.