Figure 6. Transcription of rescued genome integrated LSL-GUS gene in transgenic pBY031 Arabidopsis. Plant lines and treatments are the same as those in Figure 5. One of pBY003 (entire virus genome), pREP110 (unspliced Rep ORF), or pδREP110 (spliced Rep ORF) was included to supply Rep. The red color at 0 and 3 DAI indicates no treatment. Total RNA was purified and used for Northern blot analysis. DIG-labeled GUS specific probe was used (upper panel). The transcript signal of GUS was normalized by a small ribosomal RNA signal from the methylene blue-stained blot (lower panel) using ImageQuant™ software program. The table at bottom shows the normalized data for each treatment.