Figure 7. Rescued genome integrated LSL-GUS gene replication by transient Rep expression using Agrobacterium-mediated DNA delivery. Plant lines and treatments are described in Figure 5. Total genomic DNA was isolated, double digested with BamHI and PstI, and hybridized with GUS and Rep specific DIG-labeled cDNA probes. C. Restriction maps depict regions surrounding areas corresponding to either GUS or Rep probes. B; BamHI, P; PstI, Bar; span of cDNA probe. Lengths of restriction fragments indicated in kbp on left hand side of Figure.