Table 1. Coded levels and real values (in parentheses) for the experimental design and results of the 2^6-2 fractional factorial design.

Trial

Powered
soy (%)

Potato
starch (%)

Peptone
(%)

KH₂PO₄
(%)

MgSO₄.7H₂O
(%)

Glucose
(%)

MTGase activity (U/ml)

x₁ (X₁)^a

x₂ (X₂)

x₃ (X₃)

x₄ (X₄)

x₅ (X₅)

x₆ (X₆)

72
hrs

96
hrs

120
hrs

1

-1 (0.5)

-1 (0.5)

-1 (1.0)

-1 (0.0)

-1 (0.0)

-1 (0.1)

0.21

0.10

0.08

2

1 (2.5)

-1 (0.5)

-1 (1.0)

-1 (0.0)

1 (0.2)

-1 (0.1)

0.28

0.41

0.18

3

-1 (0.5)

1 (3.5)

-1 (1.0)

-1 (0.0)

1 (0.2)

1 (0.9)

0.02

0.51

1.08

4

1 (2.5)

1 (3.5)

-1 (1.0)

-1 (0.0)

-1 (0.0)

1 (0.9)

0.02

0.03

0.02

5

-1 (0.5)

-1 (0.5)

1 (2.0)

-1 (0.0)

1 (0.2)

1 (0.9)

0.02

0.32

0.22

6

1 (2.5)

-1 (0.5)

1 (2.0)

-1 (0.0)

-1 (0.0)

1 (0.9)

0.64

0.84

0.32

7

-1 (0.5)

1 (3.5)

1 (2.0)

-1 (0.0)

-1 (0.0)

-1 (0.1)

0.47

0.06

0.14

8

1 (2.5)

1 (3.5)

1 (2.0)

-1 (0.0)

1 (0.2)

-1 (0.1)

0.12

0.70

0.70

9

-1 (0.5)

-1 (0.5)

-1 (1.0)

1 (0.4)

-1 (0.0)

1 (0.9)

0.34

0.17

0.10

10

1 (2.5)

-1 (0.5)

-1 (1.0)

1 (0.4)

1 (0.2)

1 (0.9)

0.50

0.61

1.19

11

-1 (0.5)

1 (3.5)

-1 (1.0)

1 (0.4)

1 (0.2)

-1 (0.1)

0.63

1.15

1.14

12

1 (2.5)

1 (3.5)

-1 (1.0)

1 (0.4)

-1 (0.0)

-1 (0.1)

0.62

1.11

1.27

13

-1 (0.5)

-1 (0.5)

1 (2.0)

1 (0.4)

1 (0.2)

-1 (0.1)

0.03

0.05

0.04

14

1 (2.5)

-1 (0.5)

1 (2.0)

1 (0.4)

-1 (0.0)

-1 (0.1)

0.29

0.46

0.52

15

-1 (0.5)

1 (3.5)

1 (2.0)

1 (0.4)

-1 (0.0)

1 (0.9)

0.37

0.30

0.63

16

1 (2.5)

1 (3.5)

1 (2.0)

1 (0.4)

1 (0.2)

1 (0.9)

0.38

0.75

0.71

17

0 (1.5)

0 (2.0)

0 (1.5)

0 (0.2)

0 (0.1)

0 (0.5)

0.41

0.63

1.11

18

0 (1.5)

0 (2.0)

0 (1.5)

0 (0.2)

0 (0.1)

0 (0.5)

0.37

0.74

1.13

19

0 (1.5)

0 (2.0)

0 (1.5)

0 (0.2)

0 (0.1)

0 (0.5)

0.35

0.67

1.08