Table 1. Purification of rec-pediocin expressed in E. coli BL21.

Fraction
activity

Total activity
(AU#)

Total
protein (mg)

Specific activity
(AU/mg)

 Yield 
(%)

Fold in sp.

 Total lysate*

ND

300

ND

-

-

 Ni-NTA Eluted

4.5 x 105

15

3 x 104

100

1

 RP-HPLC fraction

2 x 105

10

2 x 104

83

1.25

#AU is defined as the mg quantity of protein require to exhibit definite zone of inhibition against the indicator bacterium as tested by spot-on-lawn assay.
*Obtained from the culture grown in 3 litre.
ND: no detection, V7 was used as an indicator strain.

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