Table 1. Chromosomal location, primer sequences and PCR conditions for microsatellite loci used in this study.

Locus

Ch¹

PCR Conditions

Primer Sequences

MgCl₂²

Annealing
Temperature (ºC)³

 BM2123

2

3.0

55

5'-GCTGCCTTCTACCAAATACCC-3'
5'-CTTCCTGAGAGAAGCAACACC-3'

 ETH10

5

3.0

55

5'-GTTCAGGACTGGCCCTGCTAACA-3'
5'-CCTCCAGCCCACTTTCTCTTCTC-3'

 INRA37

10

3.0

50

5'-GATCCTGCTTATATTTTAACCAC-3'
5'-AAAATTCCATGGAGAGAGAAAC-3'

 BM1824

1

1.2

55

5'-GAGCAAGGTGTTTTTCCAATC-3'
5'-CATTCTCCAACTGCTTCCTTG-3'

 BMS1886

2

3.0

60

5'-CAGGGACTGAAAAATAATGCC-3'
5'-TTCCATGTTGATTGTTTCTTCC-3'

 TGLA53

16

2.0

50

5'-GCTTTCAGAAATAGTTTGCATTCA-3'
5'-ATCTTCACATGATATTACAGCAGA-3'

 ILSTS005

5

0.8

50

5'-GGAAGCAATGAAATCTATAGCC-3'
5'-TGTTCTGTGAGTTTGTAAGC-3'

 BMS1987

2

3.0

60

5'-TGATGCAGAGAACGTTTTAATTT-3'
5'-CTTGGGGTAGGCAGAGATTT-3'

 INRA23

3

3.0

50

5'-GAGTAGAGCTACAAGATAAACTTC3'
5'-TAACTACAGGGTGTTAGATGAACTC-3'

 HEL5

21

1.0

50

5'-GCAGGATCACTTGTTAGGGA-3'
5'-AGACGTTAGTGTACATTAAC-3'

 TGLA44

2

3.0

65

5'-AACTGTATATTGAGAGCCTACCATG-3'
5'-CACAACTTAGCGACTAAACCACCA-3'