Table 2. ISSR primers used in the study and the number and the type of fragments they amplified.

Primer

Primer
sequence

Tm
(ºC)

bp

GC
(%)

Annealing
temp.
(ºC)

Single plant analysis

Bulk analysis

Fragment size
range (bp)

No. of
Scored
bands

No.of
polymorphic
bands

% of
polymorphic
bands

No.of
Scored
bands

No. of
polymorphic
bands

% of
polymorphic
bands

Single
plants

Bulk
plants

ISSR 1

(AGC)⁶-G

63.1

19

68.4

62

16

16

100

9

9

100

50-1600

50-1600

ISSR 2

(ACC)⁶-G

63.1

19

68.4

61

17

17

100

8

7

87.5

50-1000

50-800

ISSR 3

(AGC)⁶-C

63.1

19

68.4

62

10

7

70

9

6

66.66

50-1500

50-1500

ISSR 4

(CA)¹⁰-C

59.8

21

52.4

59

7

6

85.71

6

6

100

50-2300

50-1300

ISSR 5

(GA)⁹-C

56.7

19

52.6

56

11

11

100

8

8

100

50-1200

50-850

ISSR 6

GT-(CAC)⁷

67.8

23

65.2

66

12

12

100

8

7

87.5

50-930

50-930

ISSR 7

(AG)⁹-C

56.7

19

52.6

56

12

11

91.66

9

8

88.88

50-1000

50-1000

ISSR 8

(AC)⁹-G

56.7

19

52.6

56

12

12

100

6

6

100

50-2300

50-2300

ISSR 9

(AC)⁸-CG

56

18

55.6

55.5

11

11

100

14

14

100

50-1100

50-1250

ISSR 10

(AC)⁸-CC/T

54.8

18

52.8

55.5

19

19

100

15

13

86.66

50-1300

50-1000

ISSR 11

(CAC)⁵

53.3

15

66.7

52.5

-

-

-

2

0

0

-

300-750

ISSR 12

(GACAC)⁴

61.4

20

60

60.5

18

18

100

11

11

100

50-1300

50-1000

ISSR 13

(CA)⁶-A/GG

44.8

14

53.6

42.5

9

9

100

10

9

90

50-1200

50-1200

ISSR 14

(CA)⁶-RY

43.4

14

50

43

16

13

81.25

12

9

75

100-1100

100-2300

ISSR 15

(CA)⁸-AG

53.7

18

50

55.5

6

6

100

5

5

100

50-1250

50-950

ISSR 16

(CA)⁸-GC

56

18

55.6

55.5

18

16

88.88

11

9

81.81

50-1400

50-1000

ISSR 17

CAG-(CA)⁸

56.7

19

52.6

56

5

3

60

3

1

33.33

100-950

100-950

ISSR 18

CGT-(CA)⁸

56.7

19

52.6

56

6

5

83.33

7

5

71.42

100-780

50-1050