Table 3. Bioconversion of (+)-alpha-pinene (60 mg) by Rauvolfia sellowii (30 g fresh weight). Numbers represent mean percentages ± standard deviation (four replications) of components starting with 100% alpha-pinene at the experimental onset. |
|
Day 1 |
Day 3 |
Day 5 |
Day 7 |
Day 10 |
Day 15 |
(+)-alpha-pinene |
25.9 ± 8.3 |
3.7 ± 2.2 |
3.2 ± 1.8 |
0.0 |
0.0 |
0.0 |
trans-pinocarveol |
7.3 ± 0.5 |
8.5 ± 2.8 |
7.0 ± 3.2 |
7.5 ± 5.5 |
7.8 ± 2.9 |
12.2 ± 1.9 |
trans-verbenol |
54.5 ± 6.9 |
68.5 ± 3.0 |
61.7 ± 4.3 |
59.1 ± 4.4 |
48.0 ± 2.0 |
39.1 ± 1.5 |
myrtenol |
2.3 ± 0.7 |
3.2 ± 1.7 |
4.6 ± 1.9 |
3.8 ± 2.5 |
2.2 ± 0.8 |
2.9 ± 0.8 |
unidentified* |
2.2 ± 1.2 |
4.7 ± 3.1 |
4.8 ± 2.7 |
3.1 ± 4.2 |
3.4 ± 1.1 |
4.3 ± 3.0 |
(+)-verbenone |
7.4 ± 1.2 |
10.4 ± 2.0 |
17.9 ± 3.2 |
24.0 ± 2.1 |
32.2 ± 0.7 |
31.9 ± 3.1 |
*m/z = 95(100); 41(47.1); 93(25.2); 43(21.5); 55(17.4); 79(14.1); 67(13.9); 91(13.7); 121(13.4); 105(12.5); 53(11.6); 77(10.7); 81(7.8); 110(7.8); 139(6.5); 136(4.6); 154(2.8). |
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