Table 3. Bioconversion of (+)-alpha-pinene (60 mg) by Rauvolfia sellowii (30 g fresh weight). Numbers represent mean percentages ± standard deviation (four replications) of components starting with 100% alpha-pinene at the experimental onset.


 

Day 1

Day 3

Day 5

Day 7

Day 10

Day 15

 (+)-alpha-pinene

25.9 ± 8.3

3.7 ± 2.2

3.2 ± 1.8

0.0

0.0

0.0

 trans-pinocarveol

7.3 ± 0.5

8.5 ± 2.8

7.0 ± 3.2

7.5 ± 5.5

7.8 ± 2.9

12.2 ± 1.9

 trans-verbenol

54.5 ± 6.9

68.5 ± 3.0

61.7 ± 4.3

59.1 ± 4.4

48.0 ± 2.0

39.1 ± 1.5

 myrtenol

2.3 ± 0.7

3.2 ± 1.7

4.6 ± 1.9

3.8 ± 2.5

2.2 ± 0.8

2.9 ± 0.8

 unidentified*

2.2 ± 1.2

4.7 ± 3.1

4.8 ± 2.7

3.1 ± 4.2

3.4 ± 1.1

4.3 ± 3.0

 (+)-verbenone

7.4 ± 1.2

10.4 ± 2.0

17.9 ± 3.2

24.0 ± 2.1

32.2 ± 0.7

31.9 ± 3.1

*m/z = 95(100); 41(47.1); 93(25.2); 43(21.5); 55(17.4); 79(14.1); 67(13.9); 91(13.7); 121(13.4); 105(12.5); 53(11.6); 77(10.7); 81(7.8); 110(7.8); 139(6.5); 136(4.6); 154(2.8).


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