Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
Financial support: Consejo Nacional de Ciencia y Tecnología (CONACYT) from México (Grant No. 28643-B).
Keywords: Argemone mexicana, β-glucuronidase, benzylisoquinoline alkaloids, genetic transformation, neomycin phosphotransferase II.
Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.