Figure 7. PCR analysis of single shoots of the ‘Pinova’
transgenic apple line T357. Genomic DNA was extracted from leaf tissue of ten individual shoots:
(a) Using nptII specific primers;
(b) Using attE specific primers;
(c) Using the T-DNA specific primer pBI121_GW2 and
the primer T357_F to amplify the right border adjacent region;
(d) Using the T-DNA specific primer pBI121_GW1 and
the primer T357_R to amplify the left border adjacent region. MW 1 - 50 bp molecular
weight marker, MW 2 - 100 bp molecular weight marker (both MBI Fermentas). |