Table 1. Transgenic ‘Pinova’ apple lines: nptII and gusA activities in leaf tissue. |
Genotype |
nptII activity1 |
gusA activity4,5 of leaf number |
||||||||||
First evaluation2 |
Second evaluation3 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
T311 |
47 ± 4.0 |
3 ± 0.2 |
1 |
3 |
2 |
3 |
2 |
1 |
1 |
2 |
2 |
4 |
T315 |
24 ± 0.9 |
17 ± 0.9 |
2 |
1 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
T316 |
29 ± 0.3 |
14 ± 0.3 |
2 |
2 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
T320 |
1 ± 0.0 |
7 ± 0.4 |
2 |
1 |
1 |
2 |
1 |
2 |
2 |
1 |
2 |
1 |
T321 |
7 ± 0.2 |
17 ± 0.5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
T322 |
62 ± 5.0 |
120 ± 8.3 |
4 |
4 |
4 |
3 |
3 |
3 |
4 |
3 |
3 |
4 |
T324 |
31 ± 3.4 |
61 ± 13.6 |
3 |
3 |
3 |
4 |
3 |
3 |
4 |
5 |
3 |
3 |
T328 |
28 ± 1.1 |
10 ± 0.3 |
2 |
2 |
1 |
2 |
2 |
2 |
2 |
2 |
1 |
2 |
T329 |
30 ± 0.7 |
35 ± 2.0 |
3 |
5 |
2 |
5 |
5 |
5 |
4 |
5 |
4 |
4 |
T333 |
30 ± 1.1 |
92 ± 7.3 |
4 |
4 |
5 |
5 |
5 |
5 |
4 |
4 |
4 |
4 |
T334 |
49 ± 4.4 |
31 ± 2.2 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
T335 |
22 ± 1.7 |
21 ± 2.3 |
2 |
1 |
4 |
4 |
2 |
1 |
2 |
2 |
2 |
2 |
T336 |
18 ± 0.9 |
5 ± 0.2 |
4 |
4 |
4 |
4 |
4 |
4 |
2 |
2 |
4 |
5 |
T338 |
26 ± 1.1 |
16 ± 0.2 |
2 |
4 |
2 |
2 |
2 |
4 |
4 |
4 |
4 |
4 |
T340 |
27 ± 3.4 |
19 ± 1.7 |
4 |
4 |
5 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
T344 |
24 ± 4.4 |
11 ± 0.9 |
4 |
4 |
4 |
1 |
1 |
4 |
4 |
1 |
1 |
3 |
T345 |
13 ± 3.0 |
20 ± 2.2 |
1 |
1 |
2 |
2 |
3 |
1 |
2 |
3 |
3 |
2 |
T347 |
50 ± 1.5 |
37 ± 2.1 |
4 |
4 |
1 |
4 |
4 |
4 |
3 |
1 |
1 |
2 |
T349 |
36 ± 0.4 |
20 ± 1.1 |
1 |
1 |
1 |
1 |
2 |
1 |
4 |
1 |
1 |
1 |
T353 |
12 ± 0.4 |
46 ± 2.2 |
2 |
2 |
2 |
2 |
2 |
1 |
1 |
2 |
2 |
2 |
T355 |
2 ± 0.1 |
54 ± 0.9 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
T357 |
2 ± 0.0 |
1 ± 0.0 |
3 |
3 |
3 |
3 |
3 |
4 |
4 |
4 |
4 |
4 |
T358 |
2 ± 0.1 |
18 ± 0.6 |
2 |
1 |
2 |
1 |
2 |
2 |
1 |
1 |
2 |
1 |
T359 |
14 ± 2.8 |
54 ± 1.0 |
2 |
2 |
2 |
2 |
4 |
4 |
2 |
4 |
4 |
2 |
T360 |
9 ± 0.5 |
38 ± 2.1 |
2 |
1 |
1 |
1 |
2 |
2 |
1 |
2 |
1 |
2 |
T361 |
5 ± 0.1 |
1 ± 0.0 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
Pinova6 |
0.7 ± 0.0 |
0.6 ± 0.0 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
1NPTII protein in pg/mg fresh weight ±
SD based on ELISA. The protein activity measurement was repeated three times
for each protein sample. |
|