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| Table1. Primer sequences for cDNA synthesis and DDRT-PCR. |
Use |
Anchored Primers |
Primer Sequences |
cDNA |
DD10 |
5’ TTTTTTTTTTTG 3’ |
cDNA |
DD11 |
5’ TTTTTTTTTTTC 3’ |
cDNA |
DD12 |
5’ TTTTTTTTTTTA 3’ |
Random Decamers (Arbitrary Primers) |
Primer Sequences |
|
PCR |
A-03 |
5’ AGTCAGCCAC 3’ |
PCR |
A-09 |
5’ GGGTAACGCC 3’ |
PCR |
A-16 |
5’AGCCAGCGAA 3’ |
PCR |
A-18 |
5’AGGTGACCGT 3’ |
PCR |
A-20 |
5’ GTTGCGATCC 3’ |
PCR |
B-06 |
5’ TGCTCTGCCC 3’ |
PCR |
B-07 |
5’ GGTGACGCAG 3’ |
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