Table 2. DETs in cotton fiber and their identities.

DEGs

Primer pair

DPA

Homology with reported genes / Homology with fiber EST’s

Size & Accession # / COG or KOG (group)

0

5

10

15

20

DET 2

DD10 + A09

-

+

+

+

+

Polygalacturonase (NM118513)/GH_TM0 ovule and GA__Ed0037E01f, 7-10 DPA fiber cDNA lib. EST’s

717 bp/EF453638/Nil

DET 3

DD10 + A20

-

-

-

+

+

Uncharacterized / Nil

832 bp/EF453648/Nil

DET 4

-

+

+

+

-

RRF (YP545636)/Nil

29 bp/EF453644/Translation, ribosomal structure and biogenesis (COG0233)

DET 5

DD10 + B06

+

+

-

-

-

Uncharacterized/GH_TM0 cDNA library clone DT575216

1210 bp/EF453643/Nil

DET 6

-

-

-

+

+

endo-1,3-beta-glucanase (D88416)/ CM058G04  GH fiber cDNA Lib. EST’s

437 bp/EF453649/Nil

DET 7

DD11 + A18

+

+

-

-

-

CHS (DQ026297)/GH_TM0 ovule cDNA lib. EST’s

773 bp/EF453639/Secondary metabolites biosynthesis, transport and catabolism (COG3424)

DET 8

-

+

+

-

-

Uncharacterized/GA__Ed0037E01f , 7-10 DPA fiber lib, EST’s

248 bp/EF453647/Nil

DET 9

DD11 + B07

-

+

+

+

+

B. napus mitochondrial DNA. NADH dehydrogenase subunit I like protein (AP006444)/Nil

819 bp/EF453646/Energy production and conversion (COG1005)

DET 10

+

+

+

-

-

SPL (AJ011623) GH_TM0 ovule cDNA library clone DT556696 GA__Eb0015B06f, 7-10 DPA fiber library

443 bp/EF453640/Nil

DET 11

-

-

-

+

+

Hevea brasiliensis sucrose transporter 1 (DQ985466)/EST118 Pima 3-79 fiber normalized cDNA library Gossypium barbadense clone EE592516.

1155 bp/EF453641

Sucrose transport and related proteins (KOG0637)

DET 12

-

-

-

-

+

Erythrobacter litoralis Hypothetical Prot (YP458095)/Nil

531 bp/EF453642/Nil

DET 13

DD12 + A03

+

+

+

-

-

Arabidopsis thaliana ATOPT7 oligopeptide transporter (NM117145)/GR__Ea Gossypium raimondii cDNA clone CO100329

376 bp/EF440360/Membrane transport (KO3305, COG3104)

DET 14

-

+

+

-

-

Ribosomal L29e protein Medicago truncatula (ABE83196)/GA__Ea0007K10f Gossypium arboreum 7-10 DPA fiber library clone BE054360

286 bp/EF440361

Translation, ribosomal structure and biogenesis (KOG3504)

DET 15

-

+

+

+

-

methyl-CpG-binding domain Arabidopsis thaliana (NP563971)/GR__Ea Gossypium raimondii cDNA clone CO073108

472 bp/EF440359/Nil

DET16

DD12 + A16

+

+

+

-

-

Ribosomal protein 60S Medicago truncatula (ABE83633)/GR__Ea Gossypium raimondii cDNA clone CO085537

686 bp/EF453637

Translation, ribosomal structure and biogenesis (COG0244, KOG0815)

DET 17

-

+

+

+

-

Hypothetical protein Vitis vinifera (CAN72450)/CM072D03 Gossypium hirsutum fiber 5-10 DPA cDNA lib clone DR459063

252 bp/EF453636/Nil

DET 19

DD12 + A18

-

-

+

-

-

Arabidopsis thaliana TIP1 clone NM122042/GR__Eb Gossypium raimondii cDNA clone CO126102 GH_LSL Gossypium hirsutum cDNA clone DV851106

382 bp/EF453645 General function prediction protein (KOG0509)