Table 2. Effects of extracellular bacterial supernatants of strains of P. tunicata (Pt), H. marina (Hm), Alteromonas sp strain Ni1-LEM and Halomonas sp strain Nc1-LEM on the settlement of common biofouling diatoms and the settlement and germination of zoospores of the macroalga U. lactuca. The values presented are mean percentages of settlement in three replicate trials ± one standard deviation.


Microorganisms

Treatments

Diatoms (Bacillariophyta)

Ct SW

Ct M9

Pt

Hm

Ni1-LEM

Nc1-LEM

Nitzschia sp1

99.7 ± 8.0a

95.3 ± 12.3a

8.2 ± 0.5c

18.8 ± 1.3b

6.4 ± 1.9c*

97.2 ± 4.6a

Amphora sp

99.3 ± 6.4a

97.0 ± 8.9a

9.0 ± 1.0c

21.2 ± 3.5b

7.1 ± 0.5c*

95.2 ± 6.2a

Cylindrotheca closterium

100.0 ± 5.7a

97.9 ± 19.0a

12.2 ± 2.0c

8.6 ± 5.5c

8.0 ± 2.2c*

95.0 ± 3.0a

Nitzschia ovalis arnott

100.0 ± 9.2a

98.3 ± 6.3a

11.1 ± 2.0c

12.9 ± 3.8c

7.8 ± 1.5c*

96.6 ± 7.0a

Chaetoceros minutissimus

100.0 ± 7.5a

96.5 ± 14.9a

8.9 ± 1.2c

7.3 ± 2.1c

5.2 ± 3.0c*

95.0 ± 10.0a

Navícula sp1           

96.6 ± 8.9a

97.3 ± 15.0a

6.2 ± 4.0c

10.3 ± 4.0c

7.0 ± 6.5c*

92.9 ± 6.6a

Navícula sp2           

98.3 ± 6.4a

89.9 ± 9.3a

8.6 ± 5.0c

11.6 ± 1.6c

8.2 ± 2.0c*

93.6 ± 5.0a

Nitzschia sp2

100.0 ± 15.5a

93.9 ± 7.0a

9.2 ± 1.5c

10.4 ± 1.4c

7.7 ± 3.3c*

95.4 ± 8.8a

Algae (Chlorophyta)

Ulva lactuca

97.2 ± 12.7a

98.3 ± 8.6a

3.3 ± 4.4c

22.8 ± 2.5b

2.0 ± 0.3c*

(76.1 ± 8.2)a

(80.5 ± 9.1)a

(78.9 ± 6.9)a

(2.3 ± 0.8)c

(27.6 ± 3.3)b

(1.1 ± 0.2)c*

97.9 ± 9.3a

The values in ( ) represent percentage of germination of zoospores of U. lactuca.
Different superscripted letters indicate significant differences (one way ANOVA and LSD a posteriori test with α = 0.05 and a > b > c) of data within that row. The letter “c*” indicates the highest degree of antifouling activity.


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