Table 3. Preliminary characterization of extracellular bacterial supernatants from P. tunicata (Pt). H. marina (Hm) and Alteromonas sp (Ni1-LEM) on the settlement of the common biofouling diatoms Nitzschia sp1 (Nc), Amphora sp (Amp), Cylindrotheca closterium (Cc), Nitzschia ovalis arnott (Fp), Chaetoceros minutissimus (Nm), Navicula sp1 (Nav sp1), Navicula sp2 (Nav sp2) and Nitzschia sp2 (Nitz sp2) and in the settlement and germination of the spores of U. lactuca. The letters presented are the degree of inhibition of settlement.


Treatments

Microorganisms

 

Nc

Amp

Cc

Fp

Nm

Nav sp1

Nav sp2

Nitz sp1

U. lactuca

Enzymic

Pt + Prot

A

A

A

A

A

A

A

A

A (A)

Pt + Pept

A

A

A

A

A

A

A

A

A (A)

Hm + Prot

A

A

A

A

A

A

A

A

A (A)

Hm + Pept

A

A

A

A

A

A

A

A

A (A)

Ni1-LEM + Prot

C

C

C

C

C

C

C

C

A (A)

Ni1-LEM + Pept

A

A

A

A

A

A

A

A

A (A)

Heat denatured

Pt 80ºC

A

A

A

A

A

A

A

A

C (C)

Hm 80ºC

C

C

C

C

C

C

C

C

C (C)

Ni1-LEM 80ºC

C

C

C

C

C

C

C

C

C (C)

Dyalizates

Pt 3500 Da

C

C

C

C

C

C

C

C

C (C)

Pt 10000 Da

A

A

A

A

A

A

A

A

A (A)

Hm 3500 Da

B

B

B

B

C

B

C

C

C (C)

Hm 10000 Da

C

C

B

C

C

C

B

C

B (C)

Ni1 3500 Da

C

C

C

C

C

C

C

C

C (C)

Ni1 10000 Da

C

C

C

C

C

C

C

C

C (C)

Organic phase (non polar)

Pt

A

A

A

A

A

A

A

A

A (A)

Hm

A

A

A

A

A

A

A

A

A (A)

Ni1-LEM

A

A

A

A

A

A

A

A

A (A)

Aqueous phase (polar)

Pt

A

A

A

A

A

A

A

A

A (A)

Hm

B

B

B

B

A

A

B

A

B (B)

Ni1-LEM

A

A

A

A

A

A

A

A

A (A)

The letters in ( ) represent the percentage germination of U. lactuca zoospores. Prot: Pronase E; Pept: Carboxypeptidase G.
The level of inhibitory activity of treatments were categorize as non-inhibitory (A = 70-100% settlement), slightly inhibitory (B = 70-20% settlement) or strongly inhibitory (0-20% settlement).


Supported by UNESCO / MIRCEN network