Figure 1. Esterase polymorphisms in native PAGE and Tris-HCl, pH 8.8 for 19 individual coleoptiles (samples 1-19) obtained from C2 cycle of the popcorn composite CMS-43, showing α- and β-esterases produced from 14 loci. Two allele (1 = fast and 2 = slow) are observed in each one of the Est-1, Est-2, Est-3, Est-4, Est-5, Est-6, Est-7, Est-8, Est-9, Est-13, Est-14 loci, and the Est-10, Est-11, and Est-12 loci are monomorphics. Polymorphisms observed at the 19 samples for each locus: Est-1^1/1 (samples 5, 11, 15-16), Est-1^2/2 (samples 1-4, 6-10, 12-14, 17-19), Est-2^1/1 (samples 1, 3-13, 15-19), Est-2^1/2 (samples 2, 14), Est-3^1/1 (samples 7, 10, 17), Est-3^2/2 (sample 2), Est-3^1/2 (samples 1, 3-6, 8-9, 11-16, 18-19), Est-4^1/1 (samples 4, 9, 12), Est-4^2/2 (sample 15), Est-4^1/2 (samples 1-3, 5-8, 10-11, 13-14, 16-19), Est-5^2/2 (samples 3, 6, 9, 11-12, 16, 19), Est-5^1/2 (samples 1-2, 4-5, 7-8, 10, 13-15, 17-18), Est-6^1/1 (samples 6, 16-17), Est-6^1/2 (samples 1-5, 7-15, 18-19), Est-7^2/2 (samples 7-8), Est-7^1/2 (samples 1-6, 9-19), Est-8^1/1 (samples 3, 6-12, 19), Est-8^2/2 (sample 2), Est-8^1/2 (samples 1, 4, 5, 13-18), Est-9^1/1 (samples 6-9, 11-13, 15-16), Est-9^2/2 (samples 1, 5, 17-19), Est-9^1/2 (samples 2-4, 10, 14), Est-10, Est-11, Est-12 (samples 1-19; monomorphic), Est-13^1/1 (samples 6, 12), Est-13^1/2 (samples 1-5, 7-11, 13-19), Est-14^1/1 (samples 1-4, 11, 16, 18-19), Est-14^2/2 (samples 5, 8, 9-10, 12), Est-14^1/2 (samples 6-7, 13-15, 17).