Table 1. Infection frequency in explants of A. mexicana. Histochemical GUS assays were performed on explants twelve days after inoculation with strain LBA4404::pCAMBIA2301.


Tissue

Infected explants

GUS positive explants

Frequency of infection1

GUS foci per explant2,3

 Shoot apex

150

10

6.7

7.5 (3)a

 Leaf primordia

150

9

6.0

17.0 (6)b

 Leaves

150

6

4.0

19.3 (5)b

 Roots

150

5

3.3

2.0 (1)a

1Infection frequency was calculated as the percentage of GUS-positive explants per total number of explants examined.
2Number of GUS foci per explant was the average of GUS positive foci in at least three independent explants with standard deviation in brackets.
3Values with different letters are statistically different (P = 0.05), according to Tukey's test.


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