Figure 2. 'Osome' (Brassica rapa L.) plants transformed with pIG121Hm vector containing BAA1 gene. (a) Callus formation. (b) and (c) Multi-shoot differentiation. (d) Regenerated plants in rooting medium. Initial selection of regenerated explants by culturing on a hygromycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. (e) Acclimation of transgenic plants. (f), Obtaining of selfing seed (T1) after vernalization.


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