Figure 5. Expression of BAA1 protein in B. rapa leaf tissues of transgenic T2 lines:
a) Western blot analysis, Total soluble protein (TSP; 20 μg) was separated by 15% SDS-PAGE and transferred to membrane and immunodetected using anti-BAA polyclonal antibody. WT: wild-type; Lanes 2-9, transgenic plants.
b) ELISA analysis, WT: wild-type; 64-12-2 and 66-3-1, two transgenic plant lines.