Figure 1. ISE from Coffea arabica L. cv. Caturra and Catuaí leaves. (a) embryogenic callus (arrow). (b) Clusters of embryogenic cells (arrows) of Catuaí suspension cultures observed by light microscopy (40X). (c) Proliferation of Catuaí embryogenic calli in liquid Sli medium (d) Somatic embryo formation from Catuaí suspension culture (arrow). (e) Germination and somatic embryos conversion into plantlets in RITA^® with R liquid media. (f) Germination and somatic embryos conversion into plantlets in RITA^® with R semisolid media. (g) Coffee plants regenerated using RITA^® and semisolid culture media.