Table 5. Effect of duration of immersion period in RITA^® containers on formation of somatic embryos from suspension cultures of Coffea arabica L. cv. Catuaí after 8 weeks of culture in R medium.

Duration of immersions 

Number of somatic embryos

Fresh weight

Somatic embryos (mg)

Embryogenic callus (g)

1 min every 8 hrs

25.0 ± 2.7 a

780.0 ± 85.4 a

0.7 ± 0.2 b

5 min every 8 hrs

8.0 ± 2.0 b

249.6 ± 62.4 b

1.8 ± 0.5 ab

10 min every 8 hrs

8.2 ± 4.5 b

255.8 ± 142.1 b

2.6 ± 0.2 a