Universal protocol for generating 100bp size standard for endless usage
Neetha N. Vijayan
Linda Koshy Vaidyan
Financial support: We thank the Department of Biotechnology and Indian Council of Medical Research of Government of India for Financial support and Research fellowship.
Keywords: 100bp ladder, cost effective, DNA size standards, endless usage.
Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.