Figure 1. Schematic representation of generating 100bp ladder.
Figure 1a. Schematic representation of primers located on pGEM^®-T vector system. Arrow indicates primers and its distance in the vector backbone while the bp indicates the corresponding fragment sizes that are generated using the corresponding primers.
Figure 1b. Agarose gel picture showing the amplified PCR products of the in house ladder.
Figure 1c. Agarose gel picture showing the commercial ladder Lane M (1kb plus ladder Invitrogen) lane M1 (100bp ladder NEB) and lane M*- (In-house 100bp ladder).